Loading…

Direct Measurement of Emission of Endogenous Ozone from Plants by GC-MS-SIM

According to recent observations antibodies can catalyze the formation of previously unknown oxidants, dihydrogen trioxide (H₂O₃) and ozone (O₃), formed in the interaction between singlet oxygen (¹O₂) and H₂O. The O₃ molecules can be detected indirectly e.g. in TLC/OPLC chromatographic spots in the...

Full description

Saved in:
Bibliographic Details
Published in:Chromatographia 2010-06, Vol.71 (1), p.87-91
Main Authors: Balla, József, Tyihák, Ernő
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:According to recent observations antibodies can catalyze the formation of previously unknown oxidants, dihydrogen trioxide (H₂O₃) and ozone (O₃), formed in the interaction between singlet oxygen (¹O₂) and H₂O. The O₃ molecules can be detected indirectly e.g. in TLC/OPLC chromatographic spots in the simple BioArena operating system using O₃-eliminating molecules in the culture medium. It is currently supposed that plants can be used for the direct detection of O₃ during its eventual emission from stomata. Using the fresh primary leaves of different plant species O₃ could be really detected by means of GC-MS-SIM from the space above the the leaves which were prepared in a CO₂-containing headspace vessel. It has been also established that the CO₂ also contained O₃ molecules, and therefore, we had to use a correction to the observed data. These new findings indicate that not only formaldehyde but also O₃ and their related bioreactive compounds may play a crucial role in disease resistance (e.g. in the mechanism of action of antibodies), cell proliferation and differentiation.
ISSN:0009-5893
1612-1112
DOI:10.1365/s10337-010-1594-x