in vitro Antioxidant Activity, Total Phenolic Content and Antimicrobial Activity of Coleus forskohlii Grown in Al-Baha, Saudi Arabia

• Published in: Asian journal of chemistry 2020-08, Vol.32 (8), p.2033-2037
• Main Authors: Fadlelmula, Abdelfatah A., Al-Ghamdi, Abdulaziz Y., Abdalla, Mohamed O.M.
• Format: Article
• Language: English
• ISSN: 0970-7077; 0975-427X
• DOI: 10.14233/ajchem.2020.22716

In this study, the active ingredients from Coleus forskohlii were detected and evaluated their antioxidant and antimicrobial activities. Coleus forskohlii leaves were collected, air dried, powdered and extracted first with aqueous ethanol and secondly with petroleum ether, chloroform, ethyl acetate and n-butanol successively. The chemical analysis of the extracts showed the existence of (flavonoids, alkaloids, tannins, terpenoids, steroids, saponins and sugars). Furthermore, the extracts were subjected to analysis of total phenolic contents. Where the n-butanol exhibited highest TPC value (384.23±15.86 mg GAE/g), followed by ethyl acetate (199.73±29.35 mg GAE/g) then petroleum ether (96.97±61.29 mg GAE/g), ethanol (90.80±17.51 mg GAE/g), and eventually chloroform extract exhibited the lowest (TPC) value. In addition to that the antioxidant status of the different extracts of Coleus forskohlii was detected. Butanolic extract showed the highest radical scavenging (96.33±1.53 %, lowest IC50 value 0.03) followed by ethyl acetate extract (46.67±3.50 %, IC50 value 0.07). Eventually the antimicrobial activity tests were carried out for all the plant extracts. Where n-butanol showed the maximum inhibition (14±0.70 mm). All the pathogens except C. albicans exhibited moderate response towards petroleum ether extract. C. albicans was sensitive towards ethanol extract only (14.00±1.41), whereas the other Gram-positive and Gram-negative bacteria exhibited high impedance to the ethanol, chloroform, and ethyl acetate extracts. This study scientifically props the usage of whole plant as a medicine for several surface bacterial and fungal strike in folk medicine, in addition to this the plant may serve as a exporter for additional development of indigenous antioxidant and antitumor agents.