Long noncoding RNA H19 promotes vincristine resistance in multiple myeloma by targeting Akt

Multiple myeloma is a malignant proliferation of plasma cells that results from a single clone. Manifestations include bone pain or fractures, kidney failure, susceptibility to infection, anemia, and hypercalcemia. To investigate the relationship between vincristine (VCR) resistance and Long noncodi...

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Bibliographic Details
Published in:Cellular and Molecular Biology 2020-09, Vol.66 (6), p.76-80
Main Authors: Wang, Yigang, Xu, Shijuan, Wang, Lu, Liu, Juanni, Wang, Juan, Zhou, Jun
Format: Article
Language:English
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Summary:Multiple myeloma is a malignant proliferation of plasma cells that results from a single clone. Manifestations include bone pain or fractures, kidney failure, susceptibility to infection, anemia, and hypercalcemia. To investigate the relationship between vincristine (VCR) resistance and Long noncoding RNA H19 (Lnc-RNA H19) in multiple myeloma (MM) this experiment was set up. For this aim, RT-PCR was used to detect the expression of lnc-RNA H19 in 60 MM patients from No.215 Hospital of Shaanxi Nuclear Industry and 50 healthy controls, and further detected the expression of related genes in myeloma cell lines and VCR myeloma resistant strains. MTT assay, flow cytometry assay, western-blotting assay and luciferase assay were used to analyze the growth, apoptosis and protein phosphorylation levels of drug-resistant RPMI 8226/VCR and RPMI 8226 cells after VCR treatment and plasmid transprinting. Results showed that the relative expression of lnc-RNA H19 was significantly increased in MM patients and drug-resistant strains RPMI 8226-VCR (***p
ISSN:0145-5680
1165-158X
DOI:10.14715/cmb/2020.66.6.14