Gene transfer of {beta}2 adrenergic receptor enhances cardioprotective effects of ischemic preconditioning in rat hearts after myocardial infarction

• Published in: Interactive cardiovascular and thoracic surgery 2005-06, Vol.4 (3), p.163-167
• Main Authors: Mieno, Shigetoshi, Watanabe, Fusao, Sawa, Yoshiki, Horimoto, Hitoshi
• Format: Article
• Language: English
• ISSN: 1569-9293; 1569-9285
• DOI: 10.1510/icvts.2004.096792

a Department of Thoracic and Cardiovascular Surgery, and Chemistry, Osaka Medical College, 2-7 Daigakucho, Takatsuki, Osaka 569-8686, Japan b Department of Surgery, Course of Interventional Medicine (E1), Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan *Corresponding author. Tel.: [phone]; fax: [phone]. E-mail address : tho050{at}poh.osaka-med.ac.jp (S. Mieno). Objective: The purpose of the present study was to determine a role of ß 2 adrenergic receptor (ß 2 AR) in ischemic preconditioning (IPC) response. Methods: Post-myocardial infarction (MI) hearts were produced by ligating the left anterior descending coronary artery for 2 weeks. Post-MI hearts were transfected with the empty virus (Empty-vivo) or ß 2 AR cDNA (ß 2 AR-vivo) by intracoronary infusion of hemagglutinating virus of Japan-liposome. Empty-vivo or ß 2 AR-vivo hearts were subjected to Langendorff perfusion as Control or ß 2 AR hearts, respectively. IPC was undertaken in Control(IPC) and ß 2 AR(IPC+ß 2 AR). After global ischemia, seven hearts in each group were reperfused and normalized left ventricular peak developed pressures (LVPDP) and creatine phosphokinase (CPK) leakage were measured. ß 2 AR gene transfection was confirmed by measuring responsiveness to isoproterenol, real time RT-PCR and immunohistochemistory. Results: IPC preserved LVPDP and reduced CPK leakage in IPC+ß 2 AR hearts as compared with IPC hearts. LVPDP was decreased in addition to increase in CPK leakage in ß 2 AR hearts as compared with Control. Expression of ß 2 AR and responsiveness to isoproterenol were increased in ß 2 AR-vivo as compared with Empty-vivo hearts. Conclusion: These results indicate that ß 2 AR are required to generate IPC effects, and that ß 2 AR gene transfection enhances IPC effects in post-MI hearts. Key Words: ß 2 adrenergic receptor; Gene transfection; Ischemia; Ischemic preconditioning; Myocardial infarction