Development of a Radial Gel Diffusion Technique for the Identification of Urea in Urine Stains

A radial gel diffusion method utilizing urease and bromthymol blue has been developed for urine stain identification. Urea, present in urine in relatively high concentrations, can be detected from urine stain extracts. This technique provides both qualitative and quantitative results, and is sensiti...

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Bibliographic Details
Published in:Journal of forensic sciences 1984-04, Vol.29 (2), p.601-606
Main Authors: Bedrosian, JL, Stolorow, MD, Tahir, MA
Format: Article
Language:English
Subjects:
Forensic Medicine - methods
Humans
Immunodiffusion - instrumentation
Immunodiffusion - methods
Urea - analysis
Urine - analysis
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Summary:A radial gel diffusion method utilizing urease and bromthymol blue has been developed for urine stain identification. Urea, present in urine in relatively high concentrations, can be detected from urine stain extracts. This technique provides both qualitative and quantitative results, and is sensitive enough to detect 0.078 µg/µL of urea.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS11710J