Subtyping Phosphoglucomutase-1 in Semen Stains and Bloodstains: A Report on the Method

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The g...

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Bibliographic Details
Published in:Journal of forensic sciences 1986-10, Vol.31 (4), p.1341-1348
Main Authors: Budowle, B, Murch, RS, Davidson, LC, Gambel, AM, Kearney, JJ
Format: Article
Language:English
Subjects:
Blood Stains
Body fluids
Female
Forensic hematology
Forensic Medicine
Forensic sciences
Humans
Isoelectric Focusing
Male
Phosphoglucomutase - genetics
Semen - enzymology
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Summary:A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from semen stains and bloodstains. Isoelectric focusing of phosphoglucomutase-1 was accomplished in 80 min in a 0.2-mm-thick polyacrylamide gel with an interelectrode wick distance of 8.0 cm. The gel contained 1.2% (w/v) N-(2-hydroxyethyl) piperazine-N-3-propanesulfonic acid (EPPS) and pH 5 to 7 ampholytes (4% w/v). When maintained at room temperature, laboratory-prepared bloodstains and semen stains could be typed for phosphoglucomutase-1 up to four months and three weeks, respectively. An evaluation of phosphoglucomutase-1 typing by isoelectric focusing and the Group I system was performed on casework samples submitted to the FBI Laboratory. In addition to the increased discriminating probability of phosphoglucomutase-1 when subtyped, isoelectric focusing yielded an increase in positive calls on questioned bloodstains (65.6 versus 36.2%) and dried seminal stains (16.4 versus 13.1%) compared with the Group I system.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS11911J