A Double Origin Electrophoretic Method for the Simultaneous Separation of Adenosine Deaminase, Adenylate Kinase, and Carbonic Anhydrase II

A rapid, reliable method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II by agarose gel electrophoresis is presented. This method uses a double origin sample application system. Unreduced sample extracts for adenylate kinase analysis are applied 13...

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Bibliographic Details
Published in:Journal of forensic sciences 1986-10, Vol.31 (4), p.1349-1356
Main Authors: Murch, RS, Gambel, AM, Kearney, JJ
Format: Article
Language:English
Subjects:
Adenosine Deaminase - blood
Adenylate Kinase - blood
Blood Grouping and Crossmatching
Blood Stains
Carbonic Anhydrases - blood
Electrophoresis, Agar Gel - methods
Forensic hematology
Humans
Nucleoside Deaminases - blood
Phosphotransferases - blood
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Summary:A rapid, reliable method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II by agarose gel electrophoresis is presented. This method uses a double origin sample application system. Unreduced sample extracts for adenylate kinase analysis are applied 13.0 cm from the anode. Reduced sample extracts for the remaining proteins of interest are applied 7.0 cm from the anode. The use of applicator foils and an increased voltage gradient result in superior resolution, linearity, and band sharpness of the allozyme patterns. Further, there is no masking of the adenylate kinase 2 band as a result of the use of a reducing agent, and carbonic anhydrase II is resolved without interference from hemoglobin as has been observed with other multisystem methods.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS11912J