Population Data for Seven Y-Chromosome STR Loci from Three Different Population Groups Residing in Connecticut

The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recomme...

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Bibliographic Details
Published in:Journal of forensic sciences 2003-03, Vol.48 (2), p.1-3
Main Authors: Coyle, HM, Budowle, B, Bourke, MT, Carita, E, Hintz, JL, Ladd, C, Roy, C, Yang, NCS, Palmbach, T, Lee, HC
Format: Article
Language:English
Subjects:
Chromosomes, Human, Y
Connecticut
Continental Population Groups - genetics
DNA Fingerprinting - methods
Gene Frequency
Genetics, Population
Humans
Polymerase Chain Reaction
Tandem Repeat Sequences
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Summary:The samples for database studies were obtained from unrelated males from the indicated population groups residing in the state of Connecticut and were anonymized before analysis. The DNA from blood samples was extracted using the QIAamp® DNA blood MiniKit (Qiagen, Valencia, CA) following the recommended procedures. The quantity of human DNA was determined by slot blot hybridization using the QuantiBlot kit (Applied Biosystems, Foster City, CA) and following the manufacturer's recommended protocols. The Y-PLEX™6 system (ReliaGene Technologies, Inc., New Orleans, LA) was used for the amplification of the Y short tandem repeat (STR) loci. PCR amplification and the analysis of amplified product were performed as recommended by the manufacturer and as described in Sinha et al. (1).
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS2002345