Evaluation of Three Rapid Detection Methods for the Forensic Identification of Seminal Fluid in Rape Cases

We sought to discover whether spermatozoa concentration and the delay between ejaculation and test influence the results of seminal fluid fast detection tests. Two hundred and twenty-seven anonymous sam ples divided into four groups (normospermia, oligospermia, azoospermia, and controls) after a sem...

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Bibliographic Details
Published in:Journal of forensic sciences 2004-07, Vol.49 (4), p.1-5
Main Authors: Khaldi, N, Miras, A, Botti, K, Benali, L, Gromb, S
Format: Article
Language:English
Subjects:
Acid Phosphatase - analysis
Adult
Body fluids
Case-Control Studies
Centrifugation
Forensic Medicine - methods
Forensic sciences
Humans
Male
Middle Aged
Predictive Value of Tests
Prospective Studies
Prostate-Specific Antigen - analysis
Rape
Semen - chemistry
Semen - cytology
Sensitivity and Specificity
Spermatozoa - chemistry
Spermatozoa - cytology
Time Factors
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Summary:We sought to discover whether spermatozoa concentration and the delay between ejaculation and test influence the results of seminal fluid fast detection tests. Two hundred and twenty-seven anonymous sam ples divided into four groups (normospermia, oligospermia, azoospermia, and controls) after a semen analysis were subjected to three fast detection semen tests: Diff-Quick® fast coloration, Phosphatesmo Km Paper® for acid phosphatases (AP) detection, and PSA-Check 1® for prostate specific antigen (PSA) detection. The study was performed at three time points (0, 48, and 72 h). Unlike cytology, results obtained with AP and PSA were not influenced by spermatozoa concentration. PSA detection results remained constant up to 72 h and were more reliable after 48 h than those obtained by AP detection.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS2003317