Diabetes mellitus- and cooling-induced bladder contraction: an in vitro study

• Published in: Journal of Smooth Muscle Research 2010, Vol.46(4), pp.175-183
• Main Authors: Atalık, Kismet Esra, Okudan, Nilsel, Gokbel, Hakki, Kalkan, Serpil, Cuce, Gokhan
• Format: Article
• Language: English
• Subjects: carbachol; cooling; diabetes mellitus; potassium chloride; rat urinary bladder
• ISSN: 0916-8737; 1884-8796
• DOI: 10.1540/jsmr.46.175

The effects of diabetes mellitus during cooling on ACh- and KCl-induced responses were investigated in rat urinary bladder. Diabetes was induced in the rats by 50 mg/kg streptozotocin via an intraperitoneal injection. Rats’ body and bladder weights were measured. The isometric tension to ACh (10–9 – 3 × 10–4 M) and KCl (5–100 mM) in strips of urinary detrusor muscle of diabetic and non-diabetic rats, in organ baths at 37 and 28ºC were recorded. The body weights were significantly decreased and the bladder weights increased in STZ-induced diabetic group compared to the non-diabetic group. ACh and KCl caused concentration-dependent contractions of urinary bladders from non-diabetic and STZ-induced diabetic rats. During cooling, the sensitivity and the maximal response were significantly higher than those during 37ºC, both in non-diabetic and diabetic preparations. Cooling of detrusor muscle preparations induces a graded contraction inversely proportional to the temperature in diabetic rats. It may be assumed that the cooling response involves the same mechanisms in the diabetic and non-diabetic animals.