Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic

One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. RFLP analysis performed on 23 samples including one lab isolate showed three types of Hpa II restriction profile. A partial coat protein (CP) gene sequencing was car...

Full description

Saved in:
Bibliographic Details
Published in:Cereal research communications 2009-12, Vol.37 (4), p.541-550
Main Authors: Kundu, J.K., Jarošová, J., Gadiou, S., Červená, G.
Format: Article
Language:English
Subjects:
Agriculture
Amino acids
Barley
Capsid proteins
Cereal grains
Grains
Life Sciences
Luteovirus
Pathology
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Physiology
Plants
Polymerase chain reaction
Sequence analysis
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. RFLP analysis performed on 23 samples including one lab isolate showed three types of Hpa II restriction profile. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. One-step RT-PCR together with RFLP presents an easy and reliable assay for routine BYDV typing. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic.
ISSN:0133-3720
1788-9170
DOI:10.1556/CRC.37.2009.4.7