Identification of QTLs and associated molecular markers related to starch degradation in wheat seedlings ( Triticum aestivum L.) under saline stress

• Published in: Cereal research communications 2010-06, Vol.38 (2), p.163-174
• Main Authors: García-Suárez, J. V, J. L. Díaz de León, M. S. Röder
• Format: Article
• Language: English
• Subjects: Agriculture; biomass; chromosome mapping; Chromosomes; Genes; genetic markers; Genetics; leaf length; leaves; Life Sciences; molecular markers; Phenotypic variation; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Physiology; Plants; QTLs; Quantitative trait loci; rate of starch degradation; root length; Salinity; salt stress; Salt tolerance; Seedlings; sodium chloride; starch; Starches; Triticum aestivum; Wheat
• ISSN: 0133-3720; 1788-9170
• DOI: 10.1556/CRC.38.2010.2.2

Mapping of quantitative trait loci (QTL) was carried out in a set of 114 RILs of the International Triticeae Mapping Initiative (ITMI) mapping population under salt stress. Seedling population was grown during 8 days, under salt treatment (Hoagland’s ½ strength + 110 mM NaCl, EC 12.4 mS/cm) and normal treatment (Hoagland’s ½ strength, EC 0.9 mS/cm). We calculated starch degradation, measuring the dry weight of the grains on the 4th, 6th and 8th days of culturing. Formation of biomass was calculated measuring leaf and root length on the 4th, 6th and 8th days of culture. Interval mapping resulted in 13 QTLs, 2 major QTLs (LOD> 3) and 11 minors QTLs (LOD> 2). A total of 10 QTLs were associated with saline treatment and 3 QTLs at normal treatment. The data show that a high percentage of QTLs were in chromosomes 2B (3, 23.0%), and 1A (3, 23.0%), followed by 4D (2, 13.6%).