A Microsatellite Marker for Yellow Rust Resistance in Wheat

Bulk segregant analysis (BSA) was used to identify molecular markers associated with yellow rust disease resistance in wheat (Triticum aestivum L.). DNAs isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between yellow rust resistant and susceptible...

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Bibliographic Details
Published in:Cereal research communications 2010-06, Vol.38 (2), p.203-210
Main Authors: Akfirat, F.S., Aydin, Y., Ertugrul, F., Hasancebi, S., Budak, H., Akan, K., Mert, Z., Bolat, N., Uncuoglu, A.A.
Format: Article
Language:English
Subjects:
Agriculture
Disease resistance
Genotypes
Life Sciences
Mature plants
Microsatellite repeats
Microsatellites
Pathology
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Physiology
Rust fungi
Seedlings
Stripes
Turkeys
Wheat
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Summary:Bulk segregant analysis (BSA) was used to identify molecular markers associated with yellow rust disease resistance in wheat (Triticum aestivum L.). DNAs isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between yellow rust resistant and susceptible wheat genotypes were used to established a "tolerant" and a "susceptible" DNA pool. The BSA was then performed on these DNA pools using 230 markers that were previously mapped onto the individual wheat chromosomes. One of the SSR markers (Xgwm382) located on chromosome group 2 (A, B, D genomes) was present in the resistant parent and the resistant bulk but not in the susceptible parent and the susceptible bulk, suggesting that this marker is linked to a yellow rust resistance gene. The presence of Xgwm382 was also tested in 108 additional wheat genotypes differing in yellow rust resistance. This analysis showed that 81% of the wheat genotypes known to be yellow rust resistant had the Xgwm382 marker, further suggesting that the presence of this marker correlates with yellow rust resistance in diverse wheat germplasm. Therefore, Xgwm382 could be useful for marker assisted selection of yellow rust resistances genotypes in wheat breeding programs.
ISSN:0133-3720
1788-9170
DOI:10.1556/CRC.38.2010.2.6