A suitable solvent for adsorption of poorly water-soluble substances onto silica gel in a ready biodegradability test

When a test substance is poorly water-soluble, it can be adsorbed onto silica gel to facilitate dispersibility in a ready biodegradability test. To uniformly adsorb the test substance onto silica gel, the substance is dissolved in a solvent and then mixed with the silica gel. It is desirable for the...

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Bibliographic Details
Published in:Journal of Pesticide Science 2024, pp.D24-016
Main Authors: Takano, Yoshinari, Takekoshi, Saki, Takano, Kotaro, Matoba, Yoshihide, Mukumoto, Makiko, Sowa, Keisei, Kitazumi, Yuki, Shirai, Osamu
Format: Article
Language:English
Subjects:
adsorption solvent
metagenomic analysis
poorly water-soluble substance
ready biodegradability test
silica gel
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Summary:When a test substance is poorly water-soluble, it can be adsorbed onto silica gel to facilitate dispersibility in a ready biodegradability test. To uniformly adsorb the test substance onto silica gel, the substance is dissolved in a solvent and then mixed with the silica gel. It is desirable for the solvent to completely evaporate afterward. In this study, we identified n-hexane as a suitable solvent for this purpose. Furthermore, through fluorescence observation, we revealed that the test substance adsorbed onto the silica gel adhered to activated sludge flocs. This is thought to improve contact between the test substance and microorganisms, thereby accelerating biodegradation.
ISSN:1348-589X
1349-0923
DOI:10.1584/jpestics.D24-016