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Reaction of wheat cultivars and differential lines to Puccinia triticina races in detached leaves
The method of preserving detached wheat leaves in Petri dish was used for the inoculation and development of the fungus Puccinia triticina, the causal agent of wheat leaf rust. The reaction of 26 wheat cultivars was compared by using seedlings cultivated in pots (in vivo) and detached leaves (in vit...
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Published in: | Summa phytopathologica 2014-12, Vol.40 (4), p.353-357 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The method of preserving detached wheat leaves in Petri dish was used for the inoculation and development of the fungus Puccinia triticina, the causal agent of wheat leaf rust. The reaction of 26 wheat cultivars was compared by using seedlings cultivated in pots (in vivo) and detached leaves (in vitro) inoculated with four physiological races of the pathogen. After inoculation, the material was kept in a growth chamber for 15 days. The reaction was evaluated on the 15th day after inoculation. Results for each race in the evaluated genotypes confirmed the efficiency of the detached leaf method in assessing the reaction of wheat cultivars.
O método de preservação de folhas de trigo destacadas , em placa de Petri, foi usado para inoculação e desenvolvimento do fungo Puccinia triticina, agente causal da ferrugem da folha do trigo. Foram comparadas as reações de 26 cultivares de trigo em plântulas cultivadas em vasos (in vivo) e em folhas destacadas (in vitro) inoculadas com quatro raças fisiológicas do patógeno. Inoculou-se também 24 linhas da série diferenciadora de raças do fungo nas mesmas condições. Após a inoculação, o material foi mantido em câmara climatizada por 15 dias. A avaliação da reação foi realizada no 15º dia após a inoculação. Os resultados obtidos para cada raça nos genótipos avaliados confirmaram a eficiência do método de folha destacada na avaliação da reação de cultivares de trigo. |
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ISSN: | 0100-5405 0100-5405 |
DOI: | 10.1590/0100-5405/1993 |