Molecular cytogenetics of Dictyoloma vandellianum A. Juss. and the ancestral karyotype of Rutaceae

• Published in: Acta Botânica Brasílica 2021-12, Vol.35 (4), p.582-588
• Main Authors: Santos, Amanda, Silva, Ana Emília Barros e, Groppo, Milton, Guerra, Marcelo
• Format: Article
• Language: English
• Subjects: Aurantieae; chromosome base number; Cneoroideae; Dictyoloma vandelianum; genome size; heterochromatin; rDNA sites
• ISSN: 0102-3306; 1677-941X
• DOI: 10.1590/0102-33062020abb0450

ABSTRACT Determination of the chromosome base number of a taxon is fundamental to understanding karyotypic variation and its implications for the evolution of that group. This usually requires careful evaluation of cytological literature and robust phylogenetic support. The base number for the family Rutaceae (x = 9 or x = 18) has long been the subject of debate. Here, we analyzed the banding pattern, rDNA sites, and genome size of Dictyoloma vandellianum, subfamily Cneoroideae, the sister group of the remaining Rutaceae, and revised critical points about the chromosome base number of the family. We found that this species has n = 9, which differs from the n = 18 possessed by other cytologically known Cneoroideae species. Thus, n = 9 occurs in the main clades of Rutaceae and is the most probable base number of the family. The hypothesis of x = 18 as the base number is no longer sustainable, although n = 18 is very common in Rutaceae. Moreover, the fluorescent banding pattern and the relatively large genome size (1C = 1.3 pg) of D. vandellianum suggest that its chromosomal organization is highly divergent from Aurantieae, the only large Rutaceae clade where species with n = 9 are greatly dominant.