Biocompatibility, Biomineralization, and Maturation of Collagen by RTR®, Bioglass and DM Bone® Materials

• Published in: Brazilian dental journal 2020-09, Vol.31 (5), p.477-484
• Main Authors: Benetti, Francine, Bueno, Carlos Roberto Emerenciano, Reis-Prado, Alexandre Henrique dos, Souza, Marina Trevelin, Goto, Juliana, Camargo, Jose Maurício Paradella de, Duarte, Marco Antônio Húngaro, Dezan-Júnior, Elói, Zanotto, Edgar Dutra, Cintra, Luciano Tavares Angelo
• Format: Article
• Language: English
• ISSN: 0103-6440; 1806-4760
• DOI: 10.1590/0103-6440202003660

Abstract This study evaluated the biocompatibility, biomineralization, and collagen fiber maturation induced by Resorbable Tissue Replacement (RTR®; β-tricalcium phosphate [TCP]), Bioglass (BIOG; bioactive glass), and DM Bone® (DMB; hydroxyapatite and β-TCP) in vivo. Sixty-four polyethylene tubes with or without (control group; CG) materials (n=8/group/period) were randomly implanted in the subcutaneous tissue of 16 male Wistar rats (four per rat), weighting 250 to 280 g. The rats were killed after 7 and 30 days (n=8), and the specimens were removed for analysis of inflammation using hematoxylin-eosin; biomineralization assay using von Kossa (VK) staining and polarized light (PL); and collagen fiber maturation using picrosirius red (PSR). Nonparametric data were statistically analyzed by Kruskal-Wallis and Dunn tests, and parametric data by one-way ANOVA test (p0.05). At 30 days, there was mild inflammation in the BIOG and CG, and moderate inflammation in the RTR and DMB groups, with a significant difference between the CG and RTR (p0.05), although DMB exhibited more mature fibers than BIOG at 30 days (p