Statistical Optimisation of Fermentation Media for Novel Clostridium novyi -NT Using Response Surface Methodology

ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response...

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Bibliographic Details
Published in:Brazilian Archives of Biology and Technology 2018-01, Vol.61
Main Authors: Krishnamoorthy, Kannan, Landge, Anil Vijay
Format: Article
Language:English
Subjects:
Clostridium novyi-NT
Collagenase
Fibro-proliferative disorders
Response Surface Methodology
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Summary:ABSTRACT Clostridial collagenase is recognized as one of the important proteolytic enzymes used in treatment of varieties of fibro-proliferative disorders. The present work aimed to optimise the biomass concentration and collagenase enzyme activity of novel bacilli Clostridium novyi-NT. The response surface methodology tool was used to identify the optimal fermentation parameters. Central composite design (CCD) was applied with respect to three influencing factors - pH, proteose peptone and trypticase soya broth. These factors showed significant effect on the overall biomass concentration and collagenase enzyme activity (p
ISSN:1516-8913
1678-4324
1678-4324
DOI:10.1590/1678-4324-2018180026