Effects of budesonide and probiotics enemas on the colonic mucosa of rats with experimental colitis

• Published in: Acta cirúrgica brasileira 2007-01, Vol.22 (1), p.34-38
• Main Authors: Souza, Mardem Machado de, Aguilar-Nascimento, José Eduardo de, Gomes-da-Silva, Maria Helena, Carlos Junior, Rubens
• Format: Article
• Language: English
• Subjects: Acetic Acid; Analysis of Variance; Animals; Anti-Inflammatory Agents - therapeutic use; Biopsy; Body Weight - drug effects; Budesonide - administration & dosage; Budesonide - therapeutic use; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - genetics; Disease Models, Animal; DNA - analysis; Enema; Intestinal Mucosa - drug effects; Intestinal Mucosa - pathology; Male; Probiotics - administration & dosage; Probiotics - therapeutic use; Random Allocation; Rats; Rats, Wistar; Statistics, Nonparametric
• ISSN: 0102-8650; 0102-8650
• DOI: 10.1590/S0102-86502007000100006

To investigate the effect of enemas containing probiotics and budesonide on the colonic mucosa in experimental colitis. Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1--saline solution, group 2--budesonide (0.75 mg/kg/day), group 3--probiotics (1mg/day), group 4--probiotics plus budesonide, and group 5--control, with not-treated rats. The following variables were studied: body weight, macroscopic and microscopic score of the colonic mucosa, and DNA content of the mucosa. All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was no significant difference among the groups in relation to both the macroscopic and histological score. The budesonide + probiotic group showed higher DNA content than control group (1.24+ 0.15 versus 0.92+ 0.30 mg/100 mg of tissue, p=0.01). Budesonide in addition to probiotics enhances the mucosal trophism in experimental colitis.