Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats

PURPOSE: To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats. METHODS: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental group...

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Published in:Acta cirúrgica brasileira 2010-02, Vol.25 (1), p.117-120
Main Authors: Silva, Baldomero Antonio Kato da, Aydos, Ricardo Dutra, Silva, Iandara Schettert, Pereira, Daniel Martins, Carvalho, Paulo de Tarso Camillo de, Dourado, Doroty Mesquita, Reis, Filipe Abdalla dos, Nacer, Renato Silva
Format: Article
Language:English
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Summary:PURPOSE: To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats. METHODS: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number. RESULTS: After 16 weeks, mean of apoptotic cells number in control group (19,3±3,2) was greater than 40mg/Kg group (11,8±1,9; p
ISSN:0102-8650
0102-8650
DOI:10.1590/S0102-86502010000100023