Effect of sildenafil in renal ischemia/reperfusion injury in rats

• Published in: Acta cirúrgica brasileira 2010-12, Vol.25 (6), p.490-495
• Main Authors: Medeiros, Paulo José de, Villarim Neto, Arthur, Lima, Francisco Pignataro, Azevedo, Ítalo Medeiros, Leão, Layra Ribeiro de Sousa, Medeiros, Aldo Cunha
• Format: Article
• Language: English
• ISSN: 0102-8650; 0102-8650
• DOI: 10.1590/S0102-86502010000600006

PURPOSE: To evaluate the effect of sildenafil, administered prior to renal ischemia/reperfusion (I/R), by scintigraphy and histopathological evaluation in rats. METHODS: Twenty-four rats were divided randomly into two groups. They received 0.1 ml of 99mTechnetium-etilenodicisteine intravenous, and a baseline (initial) renal scintigraphy was performed. The rats underwent 60 minutes of ischemia by left renal artery clamping. The right kidney was not manipulated. The sildenafil group (n=12) received orally 1 mg/kg of sildenafil suspension 60 minutes before ischemia. Treatment with saline 0.9% in the control group (n=12). Half of the rats was assessed after 24 hours and half after seven days I/R, with new renal scintigraphy to study differential function. After euthanasia, kidneys were removed and subjected to histopathological examination. For statistical evaluation, Student t and Mann-Whitney tests were used. RESULTS: In the control group rats, the left kidneys had significant functional deficit, seven days after I/R, whose scintigraphic pattern was consistent with acute tubular necrosis, compared with the initial scintigraphy (p