Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor

PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal...

Full description

Saved in:
Bibliographic Details
Published in:Acta cirúrgica brasileira 2011, Vol.26 (suppl 1), p.53-56
Main Authors: Morano, José Antonio Carlos Otaviano David, Cordeiro, Naylana, Guimarães, Sergio Botelho, Jamacaru, Francisco Vagnaldo Fechine, Moraes Filho, Manoel Odorico de
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3
cites cdi_FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3
container_end_page 56
container_issue suppl 1
container_start_page 53
container_title Acta cirúrgica brasileira
container_volume 26
creator Morano, José Antonio Carlos Otaviano David
Cordeiro, Naylana
Guimarães, Sergio Botelho
Jamacaru, Francisco Vagnaldo Fechine
Moraes Filho, Manoel Odorico de
description PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P
doi_str_mv 10.1590/S0102-86502011000700011
format article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1590_S0102_86502011000700011</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1590_S0102_86502011000700011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3</originalsourceid><addsrcrecordid>eNplkN1KxDAQhYMouK4-g3mB6iRt0uRSlvUHFrxQ2cuSv7LVtClJiu7b21URwYsz58AMB-ZD6JLAFWESrp-AAC0EZ0CBEACoZxFyhBa_i-M_-RSdpfQ6X1SclAu0XX-MLna9G7LyuA_WeRxaPPkcVQrTYHHeudiHw1TjHncDjiqn2YOZvMrO4vcu7_BW-TcXC1pynKc-xHN00iqf3MWPL9HL7fp5dV9sHu8eVjebwlApctE60Ew6zpigXGoqKgDDjBCEKkNqJmujBadaCANWSG25AcNrWmpWVa1V5RLV370mhpSia5tx_kbFfUOgOfBpvvg0__iUn70iWRk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor</title><source>SciELO</source><creator>Morano, José Antonio Carlos Otaviano David ; Cordeiro, Naylana ; Guimarães, Sergio Botelho ; Jamacaru, Francisco Vagnaldo Fechine ; Moraes Filho, Manoel Odorico de</creator><creatorcontrib>Morano, José Antonio Carlos Otaviano David ; Cordeiro, Naylana ; Guimarães, Sergio Botelho ; Jamacaru, Francisco Vagnaldo Fechine ; Moraes Filho, Manoel Odorico de</creatorcontrib><description>PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P&lt;0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies. OBJETIVO: Desenvolver um modelo para avaliar os efeitos do ultra-som focal pulsado como fonte de calor para o tratamento de tumores de Walker subcutâneos implantados em ratos. MÉTODOS: Um estudo experimental, controlado, comparativo foi realizado. Vinte ratos Wistar machos (160-300 g) divididos em dois grupos (G-1: Controle e G-2: hipertermia) foram inoculados com tumor de Walker carcinossarcoma-256. Após cinco dias os ratos do grupo G-2 ratos foram submetidos a hipertermia (45ºC). O calor foi aplicado diretamente no tumor por um equipamento de ultrassonografia (3 MHz, 1,5 W/cm³). A temperatura no tumor atingiu 45ºC em 3 minutos e foi mantida nesse nível por 5 minutos. O volume do tumor foi medido nos dias 5, 8, 11, 14 e 17 após a inoculação, em ambos os grupos. Teste t não pareado foi utilizado para comparação. P &lt;0,05 foi considerado significante. RESULTADOS: O volume do tumor foi significativamente maior no 5º dia e diminuiu nos dias 11, 14 e 17 nos ratos tratados. Animais submetidos à hipertermia sobreviveram mais tempo que os animais do grupo controle. No 29º dia após a inoculação do tumor, 40% dos ratos do grupo controle e 77,78% dos ratos tratados com hipertermia permaneceram vivos. CONCLUSÃO: Os resultados obtidos mostram que o modelo proposto é bastante simples e pode ser utilizado em laboratórios menos sofisticados para estudar os efeitos da hipertermia focal no tratamento dos tumores malignos implantados ou em estudos de sobrevida.</description><identifier>ISSN: 0102-8650</identifier><identifier>EISSN: 0102-8650</identifier><identifier>DOI: 10.1590/S0102-86502011000700011</identifier><language>eng</language><ispartof>Acta cirúrgica brasileira, 2011, Vol.26 (suppl 1), p.53-56</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3</citedby><cites>FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids></links><search><creatorcontrib>Morano, José Antonio Carlos Otaviano David</creatorcontrib><creatorcontrib>Cordeiro, Naylana</creatorcontrib><creatorcontrib>Guimarães, Sergio Botelho</creatorcontrib><creatorcontrib>Jamacaru, Francisco Vagnaldo Fechine</creatorcontrib><creatorcontrib>Moraes Filho, Manoel Odorico de</creatorcontrib><title>Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor</title><title>Acta cirúrgica brasileira</title><description>PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P&lt;0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies. OBJETIVO: Desenvolver um modelo para avaliar os efeitos do ultra-som focal pulsado como fonte de calor para o tratamento de tumores de Walker subcutâneos implantados em ratos. MÉTODOS: Um estudo experimental, controlado, comparativo foi realizado. Vinte ratos Wistar machos (160-300 g) divididos em dois grupos (G-1: Controle e G-2: hipertermia) foram inoculados com tumor de Walker carcinossarcoma-256. Após cinco dias os ratos do grupo G-2 ratos foram submetidos a hipertermia (45ºC). O calor foi aplicado diretamente no tumor por um equipamento de ultrassonografia (3 MHz, 1,5 W/cm³). A temperatura no tumor atingiu 45ºC em 3 minutos e foi mantida nesse nível por 5 minutos. O volume do tumor foi medido nos dias 5, 8, 11, 14 e 17 após a inoculação, em ambos os grupos. Teste t não pareado foi utilizado para comparação. P &lt;0,05 foi considerado significante. RESULTADOS: O volume do tumor foi significativamente maior no 5º dia e diminuiu nos dias 11, 14 e 17 nos ratos tratados. Animais submetidos à hipertermia sobreviveram mais tempo que os animais do grupo controle. No 29º dia após a inoculação do tumor, 40% dos ratos do grupo controle e 77,78% dos ratos tratados com hipertermia permaneceram vivos. CONCLUSÃO: Os resultados obtidos mostram que o modelo proposto é bastante simples e pode ser utilizado em laboratórios menos sofisticados para estudar os efeitos da hipertermia focal no tratamento dos tumores malignos implantados ou em estudos de sobrevida.</description><issn>0102-8650</issn><issn>0102-8650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNplkN1KxDAQhYMouK4-g3mB6iRt0uRSlvUHFrxQ2cuSv7LVtClJiu7b21URwYsz58AMB-ZD6JLAFWESrp-AAC0EZ0CBEACoZxFyhBa_i-M_-RSdpfQ6X1SclAu0XX-MLna9G7LyuA_WeRxaPPkcVQrTYHHeudiHw1TjHncDjiqn2YOZvMrO4vcu7_BW-TcXC1pynKc-xHN00iqf3MWPL9HL7fp5dV9sHu8eVjebwlApctE60Ew6zpigXGoqKgDDjBCEKkNqJmujBadaCANWSG25AcNrWmpWVa1V5RLV370mhpSia5tx_kbFfUOgOfBpvvg0__iUn70iWRk</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Morano, José Antonio Carlos Otaviano David</creator><creator>Cordeiro, Naylana</creator><creator>Guimarães, Sergio Botelho</creator><creator>Jamacaru, Francisco Vagnaldo Fechine</creator><creator>Moraes Filho, Manoel Odorico de</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2011</creationdate><title>Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor</title><author>Morano, José Antonio Carlos Otaviano David ; Cordeiro, Naylana ; Guimarães, Sergio Botelho ; Jamacaru, Francisco Vagnaldo Fechine ; Moraes Filho, Manoel Odorico de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morano, José Antonio Carlos Otaviano David</creatorcontrib><creatorcontrib>Cordeiro, Naylana</creatorcontrib><creatorcontrib>Guimarães, Sergio Botelho</creatorcontrib><creatorcontrib>Jamacaru, Francisco Vagnaldo Fechine</creatorcontrib><creatorcontrib>Moraes Filho, Manoel Odorico de</creatorcontrib><collection>CrossRef</collection><jtitle>Acta cirúrgica brasileira</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morano, José Antonio Carlos Otaviano David</au><au>Cordeiro, Naylana</au><au>Guimarães, Sergio Botelho</au><au>Jamacaru, Francisco Vagnaldo Fechine</au><au>Moraes Filho, Manoel Odorico de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor</atitle><jtitle>Acta cirúrgica brasileira</jtitle><date>2011</date><risdate>2011</risdate><volume>26</volume><issue>suppl 1</issue><spage>53</spage><epage>56</epage><pages>53-56</pages><issn>0102-8650</issn><eissn>0102-8650</eissn><abstract>PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P&lt;0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies. OBJETIVO: Desenvolver um modelo para avaliar os efeitos do ultra-som focal pulsado como fonte de calor para o tratamento de tumores de Walker subcutâneos implantados em ratos. MÉTODOS: Um estudo experimental, controlado, comparativo foi realizado. Vinte ratos Wistar machos (160-300 g) divididos em dois grupos (G-1: Controle e G-2: hipertermia) foram inoculados com tumor de Walker carcinossarcoma-256. Após cinco dias os ratos do grupo G-2 ratos foram submetidos a hipertermia (45ºC). O calor foi aplicado diretamente no tumor por um equipamento de ultrassonografia (3 MHz, 1,5 W/cm³). A temperatura no tumor atingiu 45ºC em 3 minutos e foi mantida nesse nível por 5 minutos. O volume do tumor foi medido nos dias 5, 8, 11, 14 e 17 após a inoculação, em ambos os grupos. Teste t não pareado foi utilizado para comparação. P &lt;0,05 foi considerado significante. RESULTADOS: O volume do tumor foi significativamente maior no 5º dia e diminuiu nos dias 11, 14 e 17 nos ratos tratados. Animais submetidos à hipertermia sobreviveram mais tempo que os animais do grupo controle. No 29º dia após a inoculação do tumor, 40% dos ratos do grupo controle e 77,78% dos ratos tratados com hipertermia permaneceram vivos. CONCLUSÃO: Os resultados obtidos mostram que o modelo proposto é bastante simples e pode ser utilizado em laboratórios menos sofisticados para estudar os efeitos da hipertermia focal no tratamento dos tumores malignos implantados ou em estudos de sobrevida.</abstract><doi>10.1590/S0102-86502011000700011</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0102-8650
ispartof Acta cirúrgica brasileira, 2011, Vol.26 (suppl 1), p.53-56
issn 0102-8650
0102-8650
language eng
recordid cdi_crossref_primary_10_1590_S0102_86502011000700011
source SciELO
title Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T12%3A38%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Experimental%20model%20of%20ultrasound%20thermotherapy%20in%20rats%20inoculated%20with%20Walker-236%20tumor&rft.jtitle=Acta%20cir%C3%BArgica%20brasileira&rft.au=Morano,%20Jos%C3%A9%20Antonio%20Carlos%20Otaviano%20David&rft.date=2011&rft.volume=26&rft.issue=suppl%201&rft.spage=53&rft.epage=56&rft.pages=53-56&rft.issn=0102-8650&rft.eissn=0102-8650&rft_id=info:doi/10.1590/S0102-86502011000700011&rft_dat=%3Ccrossref%3E10_1590_S0102_86502011000700011%3C/crossref%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c298t-fe0b59e6558269b28400c5c8812ac17597cb862b88c0d89bd6c0c6723b544fda3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true