Non-linear dynamics of chromosome condensation induced by colcemid

This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the le...

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Bibliographic Details
Published in:Brazilian Archives of Biology and Technology 2013-02, Vol.56 (1), p.85-92
Main Authors: Fernandes, Thiago de Salazar e, Silva, Isvânia Maria Serafim da, Moraes, Renato Barros, Aguiar, Leandro Álvaro de Alcântara, Amaral, Ademir, Barbosa, Catão Temístocles, Nogueira, Romildo de Albuquerque
Format: Article
Language:English
Subjects:
chaos
chromosome condensation
colcemid
Non-linear dynamics
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Summary:This study investigated the dynamical process of chromosome condensation after colcemid treatment. Two pairs of human chromosomes, #2 and #3, were highlighted for the accurate identification by fluorescence in situ hybridization (FISH). A computerized image analysis system was used to measure the lengths of the two pairs of chromosomes averaged over 50 metaphases of different cultures with colcemid (0.5 µg/mL) added either at 3 or 48 h of a total 72 h culture period. For determining whether the process of chromosome condensation was chaotic or random, the algorithm of Detrended Fluctuation Analysis (DFA) was used. In order to evaluate the power of the method, the data were shuffled and DFA was performed again. It was found that colcemid prolonged treatment induced a significantly greater chromosome condensation (p
ISSN:1516-8913
1678-4324
1516-8913
DOI:10.1590/S1516-89132013000100011