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Occurrence of sulphate reducing bacteria (SRB) associated with biocorrosion on metallic surfaces in a hydroelectric power station in Ibirama (SC) - Brazil

The aim of this study was evaluate, two methods for the detection and identification of sulphate reducing bacteria (SRB): ML medium and PCR with specific primers for SRB groups. SRB were detected through the selective medium only on carbon steel, which showed corrosion. Employing specific PCR primer...

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Bibliographic Details
Published in:Brazilian Archives of Biology and Technology 2013-10, Vol.56 (5), p.801-809
Main Authors: Marangoni, Paulo Roberto Dantas, Robl, Diogo, Berton, Marcos Antonio Coelho, Garcia, Carlos Mario, Bozza, Angela, Porsani, Mariana Vieira, Dalzoto, Patricia do Rocio, Vicente, Vânia Aparecida, Pimentel, Ida Chapaval
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Language:English
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Summary:The aim of this study was evaluate, two methods for the detection and identification of sulphate reducing bacteria (SRB): ML medium and PCR with specific primers for SRB groups. SRB were detected through the selective medium only on carbon steel, which showed corrosion. Employing specific PCR primer, SBR were detected from all the metallic components assayed, even those that did not present visible corrosion spots, such stainless steel and copper alloys. Despite the presence or absence of corrosion at the later stages effectively by using the selective medium,, the initial stages of the corrosion could only be detected by the amplification of total DNA with SRB specific primers. The early detection of SRB could be employed for preventing the damages on metal surfaces before the installation of corrosion processes. Strategies for reducing the time spent on SRB isolation and identification could be auxiliary tools for controlling the corrosion of materials.
ISSN:1516-8913
1678-4324
1516-8913
DOI:10.1590/S1516-89132013000500011