Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor

The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy r...

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Bibliographic Details
Published in:Brazilian Archives of Biology and Technology 2015-04, Vol.58 (2), p.166-174
Main Authors: Habibi, Peyman, Piri, Khosro, Deljo, Ali, Moghadam, Yaser Ahmadi, Ghiasvand, Taiebeh
Format: Article
Language:English
Subjects:
Atropa belladonna
bioreactor
hairy root
scopolamine
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Summary:The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g-1 dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of minerals consumption also was at 1.25 vvm and 70 rpm. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR).
ISSN:1516-8913
1678-4324
1516-8913
DOI:10.1590/S1516-8913201400276