Variation in Apoptosis Profiles in Radiation-Induced Genomically Unstable Cell Lines

Nagar, S., Smith, L. E. and Morgan, W. F. Variation in Apoptosis Profiles in Radiation-Induced Genomically Unstable Cell Lines. Radiat. Res. 163, 324–331 (2005). Delayed reproductive cell death or lethal mutations in the survivors of irradiated cells is a well-characterized end point associated with...

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Bibliographic Details
Published in:Radiation research 2005-03, Vol.163 (3), p.324-331
Main Authors: Nagar, Shruti, Smith, Leslie E., Morgan, William F.
Format: Article
Language:English
Subjects:
Animals
Annexin A5 - chemistry
Annexins
Apoptosis
Blotting, Western
Cell culture techniques
Cell Line, Tumor
Cell lines
CHO Cells
Chromosomes, Human, Pair 4 - metabolism
Cricetinae
Cytochromes
Cytochromes c - metabolism
Delta cells
Densitometry
DNA Damage
DNA Fragmentation
Dose-Response Relationship, Radiation
Epithelial cells
Genomic Instability
Germ cells
Green Fluorescent Proteins - metabolism
Humans
Immunoprecipitation
In Situ Hybridization, Fluorescence
In Situ Nick-End Labeling
Irradiation
Metaphase
Microscopy, Fluorescence
Mitochondria - metabolism
Mitochondria - pathology
REGULAR ARTICLES
Time Factors
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Summary:Nagar, S., Smith, L. E. and Morgan, W. F. Variation in Apoptosis Profiles in Radiation-Induced Genomically Unstable Cell Lines. Radiat. Res. 163, 324–331 (2005). Delayed reproductive cell death or lethal mutations in the survivors of irradiated cells is a well-characterized end point associated with radiation-induced genomic instability. Although the mechanism for this delayed lethality has not been identified, it is thought to be a means of eliminating cells that have sustained extensive damage, thus preventing tissue disruption after radiation exposure. In this study we have tested the hypothesis that delayed reproductive cell death in chromosomally unstable GM10115 clones is due to persistently increased levels of apoptosis. Evidence for differences in apoptosis in two representative genomically unstable clones after irradiation is presented. In addition, one of the unstable clones was found to have abnormal levels of apoptosis after radiation exposure. An understanding of apoptosis in genomically unstable clones may provide insight into the maintenance of genomic instability and the mechanism by which genomically unstable cells evade cell death, potentially contributing to carcinogenesis.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR3287