Cellular distribution and function of soluble guanylyl cyclase in rat kidney and liver

Soluble guanylyl cyclase (sGC) catalyzes the biosynthesis of cGMP in response to binding of L-arginine-derived nitric oxide (NO). Functionally, the NO-sGC-cGMP signaling pathway in kidney and liver has been associated with regional hemodynamics and the regulation of glomerular parameters. The distri...

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Bibliographic Details
Published in:Journal of the American Society of Nephrology 2001-11, Vol.12 (11), p.2209-2220
Main Authors: THEILIG, Franziska, BOSTANJOGLO, Magdalena, PAVENSTÄDT, Hermann, GRUPP, Clemens, HOLLAND, Gudrun, SLOSAREK, Ilka, GRESSNER, Axel M, RUSSWURM, Michael, KOESLING, Doris, BACHMANN, Sebastian
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Guanylate Cyclase - chemistry
Guanylate Cyclase - metabolism
Investigative techniques, diagnostic techniques (general aspects)
Kidney - cytology
Kidney - enzymology
Liver - cytology
Liver - enzymology
Male
Medical sciences
Nitric Oxide - pharmacology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Solubility
Tissue Distribution
Urinary system
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Summary:Soluble guanylyl cyclase (sGC) catalyzes the biosynthesis of cGMP in response to binding of L-arginine-derived nitric oxide (NO). Functionally, the NO-sGC-cGMP signaling pathway in kidney and liver has been associated with regional hemodynamics and the regulation of glomerular parameters. The distribution of the ubiquitous sGC isoform alpha 1 beta 1 sGC was studied with a novel, highly specific antibody against the beta 1 subunit. In parallel, the presence of mRNA encoding both subunits was investigated by using in situ hybridization and reverse transcription-PCR assays. The NO-induced, sGC-dependent accumulation of cGMP in cytosolic extracts of tissues and cells was measured in vitro. Renal glomerular arterioles, including the renin-producing granular cells, mesangium, and descending vasa recta, as well as cortical and medullary interstitial fibroblasts, expressed sGC. Stimulation of isolated mesangial cells, renal fibroblasts, and hepatic Ito cells with a NO donor resulted in markedly increased cytosolic cGMP levels. This assessment of sGC expression and activity in vascular and interstitial cells of kidney and liver may have implications for understanding the role of local cGMP signaling cascades.
ISSN:1046-6673
1533-3450
DOI:10.1681/ASN.V12112209