Determination of Tetracycline and Oxytetracycline Residues in Honey by High Performance Liquid Chromatography

Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of America...

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Bibliographic Details
Published in:Journal of agricultural science and technology. B 2016-02, Vol.6 (2), p.135-139
Main Author: Shaif Mohammed Kasem Saleh Amer Mohammed Mussaed Fadhl Mohammed Al-Hariri
Format: Article
Language:English
Subjects:
反相高效液相色谱
四环素
土霉素
最大残留限量
残留量
测定
蜂蜜
高效液相色谱法
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Summary:Honey is being used as a pure natural and as an ingredient in many foods, pharmaceuticals and cosmetics than ever before, so honey testing has become essential to maintain its healthful characteristics and protect public health. Tetracyclines （TCs） are used for the treatment or prevention of American and European foul broad in bee colonies which are caused especially by two species of bacteria--Paenibacillus larvae and Melissococcus pluton. The present study aimed to determine the tetracycline residues in different types of local and imported honey. The reversed-phase high performance liquid chromatography （HPLC） with ultraviolet detection （UV） was used. Tetracycline and oxytetracycline were extracted from honey with 0.01 M sodium succinate buffer. Of the examined samples, 31.25% and 12.50% had these oxytetracycline and tetracycline residues, respectively. It was observed that all the samples studied have been reported residues of tetracycline and oxytetracycline more than international standards of maximum residue limit （MRLs）.
ISSN:2161-6264
2161-6264
DOI:10.17265/2161-6264/2016.02.009