Anti-Leishmanial and Immunomodulatory Effects of Epigallocat-echin 3-O-Gallate on Leishmania tropica: Apoptosis and Gene Expression Profiling

Background: Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA agains...

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Published in:Iranian journal of parasitology 2019-12
Main Authors: SADUQI, Morteza, SHARIFI, Iraj, BABAEI, Zahra, KEYHANI, Alireza, MOSTAFAVI, Mahshid, HAKIMI PARIZI, Maryam, GHASEMIAN, Mahdiyeh, BAMOROVAT, Mehdi, SHARIFI, Fatemeh, AFLATOONIAN, Mohammad Reza, SHARIFIFAR, Fariba, GHASEMI NEJAD, Pooya, KHOSRAVI, Ahmad, SALARKIA, Ehsan, VARMA, Rajender S.
Format: Article
Language:English
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Summary:Background: Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against Leishmania tropica stages. Methods: All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. Results: Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated (P
ISSN:1735-7020
DOI:10.18502/ijpa.v14i4.2094