Recent Bayesian stable-isotope mixing models are highly sensitive to variation in discrimination factors

• Published in: Ecological applications 2011-06, Vol.21 (4), p.1017-1023
• Main Authors: Bond, Alexander L, Diamond, Antony W
• Format: Article
• Language: English
• Subjects: Animals; Applied ecology; assumptions; Bayes Theorem; Birds - metabolism; Carbon - metabolism; Carbon Isotopes - metabolism; Communications; conservation; Diet; discrimination factor; Ecological modeling; Ecology; Fishes - metabolism; Food Chain; Isotope ratios; Isotopes; management; Marine; Marine ecology; mixing model; MixSIR; Modeling; Models, Biological; Nitrogen - metabolism; Nitrogen Isotopes; Puffinus mauretanicus; Sea birds; sensitivity analysis; SIAR; stable isotope; Sterna hirundo; Wildlife ecology
• ISSN: 1051-0761; 1939-5582
• DOI: 10.1890/09-2409.1

Stable isotopes are now used widely in ecological studies, including diet reconstruction, where quantitative inferences about diet composition are derived from the use of mixing models. Recent Bayesian models (MixSIR, SIAR) allow users to incorporate variability in discrimination factors (ΔΔ 13 C or ΔΔ 15 N), or the amount of change in either δδ 13 C or δδ 15 N between prey and consumer, but to date there has been no systematic assessment of the effect of variation in ΔΔ 13 C or ΔΔ 15 N on model outputs. We used whole blood from Common Terns ( Sterna hirundo ) and muscle from their common prey items (fish and euphausiids) to build a series of mixing models in SIAR (stable isotope analysis in R) using various discrimination factors from the published literature for marine birds. The estimated proportion of each diet component was affected significantly by ΔΔ 13 C or ΔΔ 15 N. We also use recently published stable-isotope data on the reliance of critically endangered Balearic Shearwaters ( Puffinus mauretanicus ) on fisheries discards to show that discrimination factor choice can have profound implications for conservation and management actions. It is therefore crucial for researchers wishing to use mixing models to have an accurate estimate of ΔΔ 13 C and ΔΔ 15 N, because quantitative diet estimates can help to direct future research or prioritize conservation and management actions.