Glucose Sensing Using Glucose Oxidase-Glutaraldehyde-Cysteine Modified Gold Electrode

The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast elec...

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Bibliographic Details
Published in:International journal of electrochemical science 2017-07, Vol.12 (7), p.5806-5817
Main Authors: Lović, J., Stevanović, S., Nikolić, N.D., Petrović, S., Vuković, D., Prlainović, N., Mijin, D., Ivić, M. Avramov
Format: Article
Language:English
Subjects:
Biosensors
Enzyme catalysis
Immobilization
Self-assembly
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Summary:The method to develop a stable glucose biosensor with successive attachment of cysteine (Cys), glutaraldehyde (GA) and glucose oxidase (GOx) onto gold electrode is presented. The cyclic voltammetry (CV) suggests the diffusion control of the glucose oxidation. The obtained biosensor shows a fast electron transfer of k0 = 20.4 s-1, high affinity for glucose with the apparent Michaelis-Menten constant KMapp= 1.15 mM, a low detection limit of 0.94 mM in a linear range 1.5-7 mM. This biosensor exhibits good stability and reproducibility. Good biocompatibility of modified electrode surface, which enhances the covalent bonded enzyme and consequently glucose oxidation, resulted in biosensor with excellent performances. Biosensor was tested in samples containing human serum.
ISSN:1452-3981
1452-3981
DOI:10.20964/2017.07.65