Development of A Fast Method for Fructus Aurantii Identification by Electrochemical Fingerprint

Electrochemical analysis techniques can be used for the identification of plant samples. This work describes the identification of fructus aurantii and its closely related species by electrochemical fingerprinting. For a better extraction of electrochemically active substances, DMSO-CHCl3-CH3OH, 2:2...

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Bibliographic Details
Published in:International journal of electrochemical science 2022-11, Vol.17 (11), p.221176, Article 221176
Main Authors: Cen, Yi, Shen, Changming, Zheng, Xiaorong, Li, Junfei, Jiang, Jianwei
Format: Article
Language:English
Subjects:
Feature extraction
Fructus aurantii. Electrochemical fingerprints
Pattern recognition
Plant identification
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Summary:Electrochemical analysis techniques can be used for the identification of plant samples. This work describes the identification of fructus aurantii and its closely related species by electrochemical fingerprinting. For a better extraction of electrochemically active substances, DMSO-CHCl3-CH3OH, 2:2:1, v/v was used as a solvent. Electrochemical fingerprints were collected in two different buffer solutions. The collected fingerprint profiles can be used for density plots construction. The plants can be automatically identified by feature extraction of density plot. The oxidation peaks exhibited in the electrochemical fingerprint are most likely the oxidation of narirutin, naringin, hesperidin, and neohesperidin. Therefore, HPLC was used for the validation of the standards and samples. Finally, electrochemical techniques were used to document the electrochemical behavior of these four substances. The results suggest that narirutin, naringin, hesperidin, and neohesperidin may be the most significant substances contributing to the electrochemical fingerprinting of fructus aurantii.
ISSN:1452-3981
1452-3981
DOI:10.20964/2022.11.66