IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THE COMPONENTS OF AMOMUM TSAO-KO IN MURINE MACROPHAGE RAW 264.7 CELLS

• Published in: African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2018-11, Vol.15 (2), p.26-34
• Main Authors: Choi, Chun Whan, Shin, Ju Young, Seo, Changon, Hong, Seong Su, Ahn, Eun-Kyung, Jung, Young Hoon, Oh, Joa Sub
• Format: Article
• Language: English
• Subjects: Amomum tsao-ko; Anti-inflammatory activity; Cytokines; NF-κB; Phytochemical
• ISSN: 0189-6016; 2505-0044
• DOI: 10.21010/ajtcam.v15i2.4

Background: Plants still remain the prime source of drugs for the treatment of inflammation and can provide leads for the development of novel anti-inflammatory agents. Material and methods: An in vitro bioassay guide revealed that 80% ethanol (EtOH) extract of the whole plant, Amomum tsao-ko (Zingiberaceae), displayed anti-inflammatory activity after assessing its effects on murine macrophage RAW 264.7 cells. Result: Phytochemical study of the 80% EtOH extract of Amomum tsao-ko led to the isolation of eight compounds: 4-hydroxy-3-methoxy-benzoic acid ( 1 ), meso-hannokinol ( 2 ), (+)-hannokinol ( 3 ), coumaric acid ( 4/b>), 4-hydroxy-benzoic acid ( 5 ), (+)-epicatechin ( 6 ), (-)-catechin ( 7 ), and myrciaphenone A ( 8 ). The results indicated that two of the isolated components, (+)-epicatechin ( 6 ) and (-)-catechin ( 7 ), inhibited the production of nitric oxide (NO) significantly in lipopolysaccharide treated RAW 264.7 cells. Conclusion: LPS-induced interleukin tumor necrosis factor-alpha (TNF-α), IL-1β and IL-10 production was also decreased in a dose-dependent manner. In addition, wester blot analysis revealed that (+)-epicatechin ( 6 ) and (-)-catechin ( 7 ) reduced the expression of inducible nitric oxide synthase and inhibited nuclear localization of nuclear factor kappa-B (NF-κB).