APPLICATIONS OF CAPILLARY ELECTROPHORESIS TO REDOX PROTEINS AND NUCLEOTIDES IN PHOTOSYNTHETIC SYSTEM

Several redox proteins and nucleotides involved in photosynthetic electron transfer were separated by capillary zone electrophoresis (CZE) with a noncoated fused silica capillary column. We found that reduced and oxidized forms of plastocyanin, cytochrome c, NAD and NADP could be separated by CZE. T...

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Bibliographic Details
Published in:Analytical Sciences 1991, Vol.7(Supple), pp.1545-1548
Main Authors: SENDA, MASAAKI, SASAKI, TORU, NAKAMOTO, HITOSHI, HIYAMA, TETSUO
Format: Article
Language:English
Subjects:
Capillary electrophoresis
membrane proteins
nicotinamide nucleotides
photosynthesis
redox proteins
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Summary:Several redox proteins and nucleotides involved in photosynthetic electron transfer were separated by capillary zone electrophoresis (CZE) with a noncoated fused silica capillary column. We found that reduced and oxidized forms of plastocyanin, cytochrome c, NAD and NADP could be separated by CZE. Two types of ferredoxin, commonly present among higher plants, were also clearly separated by this technique.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.7.Supple_1545