COLLABORATIVE WORK TO EVALUATE TOXICITY ON MALE REPRODUCTIVE ORGANS BY REPEATED DOSE STUDIES IN RATS : 1)EFFECTS OF 2-WEEKS DAILY ADMINISTRATION OF ESTRADIOL BENZOATE

As part of a collaborative project to assess whether a 2-weeks administration period is sufficient to detect testicular toxicity of various compounds, male rats were subcutaneously administered 0, 5, 20, 50 or 100μg/kg of estradiol benzoate (E2B), a known testicular toxicant, daily for 2 or 4 weeks....

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Bibliographic Details
Published in:Journal of toxicological sciences 2000/10/25, Vol.25(SpecialIssue), pp.23-31
Main Authors: HATA, Junko, TAKAHASHI, Hiroyuki, NAKAHARA, Chiho, NAMIKI, Ayumi, UNO, Hiroshi, ASANO, Satoshi
Format: Article
Language:English
Subjects:
Adrenal Glands - drug effects
Adrenal Glands - pathology
Animals
Biological and medical sciences
Body Weight - drug effects
Chemical and industrial products toxicology. Toxic occupational diseases
Dose-Response Relationship, Drug
Estradiol - administration & dosage
Estradiol - analogs & derivatives
Estradiol - toxicity
Genitalia, Male - drug effects
Genitalia, Male - pathology
Genitalia, Male - physiopathology
ICH
Injections, Subcutaneous
Male
Medical sciences
Organ Size - drug effects
Pituitary Gland - drug effects
Pituitary Gland - pathology
Rats
Rats, Sprague-Dawley
Specific Pathogen-Free Organisms
Testis - drug effects
Testis - pathology
Time Factors
Toxicity Tests
Toxicology
Various organic compounds
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Summary:As part of a collaborative project to assess whether a 2-weeks administration period is sufficient to detect testicular toxicity of various compounds, male rats were subcutaneously administered 0, 5, 20, 50 or 100μg/kg of estradiol benzoate (E2B), a known testicular toxicant, daily for 2 or 4 weeks. After 4-weeks, suppression of body weight gain, increase in the weight of the adrenal gland, and gross changes such as decrease in size of the prostate and seminal vesicles, and increase in size of the adrenal gland were observed in the 5, 20, 50 and 100μg/kg groups. On histopathological examination, degeneration/necrosis of Pachytene spermatocytes, atrophy of Leydig cells, mature spermatid retention (Lee, et al., 1993) at stages IX, X and XI, and atrophy of ducts of the epididymides were also observed in the 5, 20, 50 and 100μg/kg groups After 2-weeks, the same changes were also observed with 20, 50 and 100μg/kg, but not 5μg/kg. These results indicate that the toxic effects of E2B are detectable by administration for 2 weeks at an appropriate dose level.
ISSN:0388-1350
1880-3989
DOI:10.2131/jts.25.SpecialIssue_23