Phylogenetic Analysis and Expression Profiles of Starch Phosphorylase Family Genes in Cassava under Diverse Environments

• Published in: Crop science 2018-05, Vol.58 (3), p.1181-1191
• Main Authors: Pei, Jinli, Zhang, Shengkui, Xia, Zhiqiang, Chen, Xin, Liu, Chen, Zhou, Xincheng, Ma, Pingan, Wang, Wenquan
• Format: Article
• Language: English
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci2017.10.0614

Starch phosphorylase (Pho), especially the plastidial type, plays an important role in starch biosynthesis. The availability of a complete cassava (Manihot esculenta Crantz) genome database allowed the identification of five Pho family members, which could be grouped into the plastidial type (MePho1a, MePho1b, and MePho1c) and cytosolic type (MePho2 and MePho3) on the basis of phylogenetic analysis. Spatial gene expression analysis of various cassava tissues revealed that plastidial MePho1b had the highest transcriptional activity in root stele, whereas the cytosolic MePho2 was expressed with higher levels in leaves and petiole. Spatial expression analysis showed that MePho1b was strongly expressed in the tuber during root development process, especially at 70 and 180 d after planting, whereas MePho1a had highest expression in leaves at 180 d after planting. Expression of MePho1b and MePho2 could be induced by salt, drought, exogenous abscisic acid, methyl jasmonate, and salicylic acid signal in the leaves of abiotic stress‐treated plantlets. In addition, the expression profiles of MePho1b and starch branching enzyme gene member MeSBE2.2 exhibited significant correlation under diverse stress environments, indicating that they may have coordinated functions.