Antioxidative stress effects of vitamins C, E, and B 12 , and their combination can protect the liver against acetaminophen-induced hepatotoxicity in rats

Several vitamins, including C, E, and B , have been recognized as antioxidants and have shown hepatoprotective effects against the hepatotoxicity caused by acetaminophen (APAP) overdose. The current investigation aims to study the effect of these vitamins and their combination in protecting the live...

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Bibliographic Details
Published in:Drug design, development and therapy development and therapy, 2018-01, Vol.12, p.3525-3533
Main Authors: Abdulkhaleq, Farah M, Alhussainy, Tawfiq M, Badr, Mujtaba M, Khalil, Asad A Abu, Gammoh, Omar, Ghanim, Bayan Y, Qinna, Nidal A
Format: Article
Language:English
Subjects:
Acetaminophen
Animals
Antioxidants - administration & dosage
Antioxidants - pharmacology
Ascorbic Acid - administration & dosage
Ascorbic Acid - pharmacology
Chemical and Drug Induced Liver Injury - drug therapy
Chemical and Drug Induced Liver Injury - pathology
Liver - drug effects
Liver - pathology
Male
Oxidative Stress - drug effects
Rats
Rats, Sprague-Dawley
Vitamin B 12 - administration & dosage
Vitamin B 12 - pharmacology
Vitamin E - administration & dosage
Vitamin E - pharmacology
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Summary:Several vitamins, including C, E, and B , have been recognized as antioxidants and have shown hepatoprotective effects against the hepatotoxicity caused by acetaminophen (APAP) overdose. The current investigation aims to study the effect of these vitamins and their combination in protecting the liver from APAP hepatotoxicity in rats. An in vitro model of freshly isolated rat hepatocytes was utilized for assessing hepatocyte mitochondrial activity conducted by cell proliferation assay (MTT). The isolated hepatocytes were treated with vitamin C, vitamin E, vitamin B and their combination, with and without further addition of toxic concentrations of APAP. In addition, an in vivo experiment was carried out on Sprague Dawley rats treated intraperitoneally for 8 days with emulsions of the vitamins or their combination prior to injecting them with APAP. In vitro results showed that vitamins C and B and the combination preparation significantly increased the percentage of hepatocyte mitochondrial activity, both with and without the addition of APAP (
ISSN:1177-8881
1177-8881
DOI:10.2147/DDDT.S172487