Vitamin D 3 Supplementation Promotes Regulatory T-Cells to Maintain Immune Homeostasis After Surgery for Early Stages of Colorectal Cancer

• Published in: In vivo (Athens) 2023-01, Vol.37 (1), p.286-293
• Main Authors: Srichomchey, Patnapa, Sukprasert, Sophida, Khulasittijinda, Nathapong, Voravud, Narin, Sahakitrungruang, Chucheep, Lumjiaktase, Putthapoom
• Format: Article
• Language: English
• Subjects: Cholecalciferol - administration & dosage; Colorectal Neoplasms - immunology; Colorectal Neoplasms - surgery; Dietary Supplements; Homeostasis; Humans; Interleukin-10 - immunology; Leukocytes, Mononuclear - immunology; T-Lymphocytes, Regulatory - immunology; Transforming Growth Factor beta1 - immunology
• ISSN: 0258-851X; 1791-7549
• DOI: 10.21873/invivo.13078

Vitamin D (VD ) affects the regulation of the immune system, including the differentiation and function of regulatory T-cells (Tregs). Tregs play an important role in maintaining immune homeostasis in patients with colorectal cancer (CRC). The effects of VD on Treg-associated immune function were investigated in Thai patients in the early stages of CRC. Twenty-eight patients were randomized to one of two groups: Untreated or treatment with VD for 3 months. Whole blood samples were collected at baseline, and at 1 and 3 months. Peripheral blood mononuclear cells were isolated and the populations of forkhead box P3-positive Treg cells was analyzed by flow cytometry. The levels of Treg-associated cytokines, interleukin 10 (IL-10) and transforming growth factor beta 1 (TGF-β1), were measured by enzyme-linked immunosorbent assays. Serum VD levels of the VD -treated group were significantly increased at 1 (p=0.017) and 3 months (p