NARINGENIN-LOADED D-Α-TOCOPHERYL POLYETHYLENE GLYCOL SUCCINATE 1000 POLYMERIC NANOSUSPENSION: AN IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITY

• Published in: Asian journal of pharmaceutical and clinical research 2019-01, p.459-463
• Main Authors: RAJAMANI, SUMATHI, KALYANA SUNDARAM, GOBINATH, SENGODAN, TAMIZHARASI, THANGAVELU, SIVAKUMAR, SHANMUKHAN, NIKHITHA K, RADHAKRISHNAN, ARUN
• Format: Article
• Language: English
• ISSN: 0974-2441; 0974-2441
• DOI: 10.22159/ajpcr.2019.v12i2.29331

Objective: Naringenin (NAR) a flavonoid, exhibits extensive pharmacological action, fails to attain a significance in application due to low aqueous solubility (~ 0.214 mg/mL) which results in low bioavailability (5.8%). Nanosuspension of NAR (NARNS) was prepared in our previous studies using high-pressure homogenization employing various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in vitro and in vivo methods. Methods: Denaturation of protein method and membrane stabilization methods was chosen for in vitro evaluation. In vivo studies performed were acute inflammatory studies (carrageenan-induced paw edema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats. Results: The studies demonstrated that the NAR and NARNS at a dose of 50mg/kg P.O. have a potent activity compared to the standard drug diclofenac. Conclusion: The percentage of protection against inflammation exhibited by NARNS was highly significant compared to NAR.