PIPERACILLIN ESTIMATION BY ION-ASSOCIATIVE COMPLEX FORMATION

Objective: The objective of the study was to develop a simple, validated, and affordable visible spectrophotometric method for determination of piperacillin (PIP) present in bulk and powder for injection formulation. Methods: In the present method, cobalt thiocyanate (CTC) was used as a chromogenic...

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Bibliographic Details
Published in:Asian journal of pharmaceutical and clinical research 2019-02, p.159-163
Main Authors: GORUMUTCHU, GIRI PRASAD, RATNAKARAM, VENKATA NADH, KUMAR KATARI, KIRAN
Format: Article
Language:English
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Summary:Objective: The objective of the study was to develop a simple, validated, and affordable visible spectrophotometric method for determination of piperacillin (PIP) present in bulk and powder for injection formulation. Methods: In the present method, cobalt thiocyanate (CTC) was used as a chromogenic reagent where it forms 2:1 ion pair complex at pH 2 with PIP which is having secondary and tertiary amine groups. Results: The formed bluish-green colored ion pair between PIP and CTC is quantitatively extractable into nitrobenzene with an absorption maximum of 665 nm. Regression analysis (r=0.9996) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (3–18 μg/mL). Low values of relative standard deviation (
ISSN:0974-2441
0974-2441
DOI:10.22159/ajpcr.2019.v12i3.29289