ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF ERUCA SATIVA (JARJEER) LEAVES EXTRACT ON CARCINOMA OF WOMEN’S BREAST

• Published in: Asian journal of pharmaceutical and clinical research 2021-01, p.89-92
• Main Authors: A DERBALA, SAFAA, E ELKADY, MANAR, A ELBANHAWY, REHAB, AF, ABDEL-AZIZ
• Format: Article
• Language: English
• ISSN: 0974-2441; 0974-2441
• DOI: 10.22159/ajpcr.2021.v14i2.39337

Objective: This work aims to investigate the influence of Eruca sativa leaves extract on the cell viability of the breast carcinoma cell line (MCF-7). Methods: In vitro, breast cancer cell line (MCF-7) treated by E. sativa leaves extract for 48 h. The cell viability, proliferation, and apoptosis were assessed using colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric technique, and antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT]) measurement. Results: This study demonstrated that the incubation of MGF-7 cells with E. sativa for 48 h caused a significative reduction in cell viability and proliferation of MGF-7 cell line. In parallel, E. sativa treatment induces a significant increase in apoptosis of MGF-7 cells compared to control. Moreover, flow cytometry analysis demonstrated that the inhibition of MGF-7 cell proliferation existed at the G2 and M phase in the cell-division cycle. Finally, the intracellular antioxidant enzymes SOD and CAT activities were significantly increased in the administered cells compared with unadministered MCF-7 cells. Conclusions: Taken together, E. sativa treatment reduces cell viability and proliferation concomitant with enhanced antioxidant enzymes expression and apoptosis of breast cancer cell line MGF-7. This may help in protection from breast cancer or preclinical recommendation.