m 6 A-mediated upregulation of lncRNA RMRP boosts the progression of bladder cancer via epigenetically suppressing SCARA5

This study aimed to elucidate the relationship between and in bladder cancer and their underlying mechanism. Biological functions were evaluated using cell-counting kit 8 assay, 5-ethynyl-2'-deoxyuridine incorporation, wound healing and Transwell assays. RNA immunoprecipitation, RNA pull-down a...

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Bibliographic Details
Published in:Epigenomics 2023-04, Vol.15 (7), p.401-415
Main Authors: Lu, Xinsheng, Chen, Libo, Liu, Shucheng, Cao, Youhan, Huang, Zhongxin
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Cell Proliferation
Gene Expression Regulation, Neoplastic
Humans
Methyltransferases - genetics
Methyltransferases - metabolism
Mice
Mice, Nude
MicroRNAs - metabolism
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Scavenger Receptors, Class A - genetics
Scavenger Receptors, Class A - metabolism
Transcriptional Activation
Up-Regulation
Urinary Bladder Neoplasms - genetics
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Summary:This study aimed to elucidate the relationship between and in bladder cancer and their underlying mechanism. Biological functions were evaluated using cell-counting kit 8 assay, 5-ethynyl-2'-deoxyuridine incorporation, wound healing and Transwell assays. RNA immunoprecipitation, RNA pull-down and chromatin immunoprecipitation were employed. A xenograft tumor model in nude mice was also conducted. and exhibited an inverse correlation. Downregulation of significantly suppressed bladder cancer cell proliferation, migration and invasion, which was reversed by overexpression. recruited DNA methyltransferases to the promoter region of , thereby triggering the methylation of the promoter to epigenetically suppress its expression. Our findings elucidate the machinery by which , stabilized by METTL3, exerts a promoter role in bladder cancer tumorigenesis by triggering methylation.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2023-0062