Estimation of Pralatrexate by Simple and Fast Reverse Phase High Performance Liquid Chromatographic Method

A simple, specific, precise, accurate, and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the determination of Pralatrexate in both pure and pharmaceutical dosage forms. In this method Agilent (4.6×150mm) 5µ column in isocratic mode with mobile phase co...

Full description

Saved in:
Bibliographic Details
Published in:International journal for research in applied science and engineering technology 2022-09, Vol.10 (9), p.840-843
Main Authors: Kulsum, Syeda, Naz, Ayesha, Zareen, Misbah, Noor, Amatul
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple, specific, precise, accurate, and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the determination of Pralatrexate in both pure and pharmaceutical dosage forms. In this method Agilent (4.6×150mm) 5µ column in isocratic mode with mobile phase containing water: methanol (25:75% v/v) was selected. The effluents were monitored at 300 nm and flow rate was fixed as 1.4 ml / min. The retention time was 3.312 min. The linearity was in the range of 20-100 g / ml. This method was validated for linearity, precision, limit of detection, limit of quantification and accuracy. Statistical analysis proves that the method is precise, reproducible and selective for the estimation of the pralatrexate drug
ISSN:2321-9653
2321-9653
DOI:10.22214/ijraset.2022.46716