Dynamic Confocal Imaging of Mitochondria in Swimming Tetrahymena and of Microtubule Poleward Flux in Xenopus Extract Spindles

Maddox et al report the use of a real-time, spinning-disk confocal scanning unit mounted on a Leica microscope to investigate the internal dynamics of fluorescently labeled microtubules in mitotic spindles. They aimed to test the spatial and temporal resolution and the optical sectioning capability...

Full description

Saved in:
Bibliographic Details
Published in:The Biological bulletin (Lancaster) 1999-10, Vol.197 (2), p.263-265
Main Authors: Maddox, Paul, Desai, Arshad, Salmon, E. D., Mitchison, T. J., Oogema, Karen, Kapoor, Tarun
Format: Article
Language:English
Subjects:
Animals
Cell Motility
Cellular biology
Fluorescence
Image processing
Imaging
Interphase
Marine biology
Methods
Microfilaments
Microscopes
Microscopy, Confocal - methods
Microtubules
Mitochondria
Mitochondria - ultrastructure
Mitotic spindle apparatus
Oocytes
Spindle Apparatus - ultrastructure
Spindles
Swimming
Tetrahymena - ultrastructure
Vanadates
Xenopus laevis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Maddox et al report the use of a real-time, spinning-disk confocal scanning unit mounted on a Leica microscope to investigate the internal dynamics of fluorescently labeled microtubules in mitotic spindles. They aimed to test the spatial and temporal resolution and the optical sectioning capability of the microscope setup.
ISSN:0006-3185
1939-8697
DOI:10.2307/1542641