Phenotypic expression of recombinant plasmids pKT107 and pHK11 in an avirulent avian Escherichia coli

An avirulent wild-type avian Escherichia coli strain (Av) was electrotransformed with plasmids coding for complement resistance (pKT107) and Colicin V (ColV) production (pHK11) in order to study the effects of complement resistance and ColV production on virulence. Transformants were also compared w...

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Bibliographic Details
Published in:Avian diseases 1994-01, Vol.38 (1), p.127-134
Main Authors: Wooley, R.E, Nolan, L.K, Brown, J, Gibbs, P.S, Bounous, D.I
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - toxicity
Antibiotic resistance
Blotting, Southern
Chick Embryo
Chickens
Colicins - biosynthesis
Complement System Proteins - physiology
DNA
DNA Probes
Drug Resistance, Microbial - genetics
Embryos
ESCHERICHIA COLI
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - pathogenicity
FACTEUR IMMUNOLOGIQUE
FACTORES INMUNOLOGICOS
FAGOCITOS
Genes, Bacterial
Guinea Pigs
HEMOLISIS
HEMOLYSE
INDICE DE CRECIMIENTO
MACROFAGOS
MACROPHAGE
Macrophages
Microbial Sensitivity Tests
MOUVEMENT
MOVIMIENTO
PHAGOCYTE
Phagocytosis
PLASMIDE
PLASMIDIOS
Plasmids
PODER PATOGENO
POLLITO
POUSSIN
POUVOIR PATHOGENE
R Factors
Recombination, Genetic
TAUX DE CROISSANCE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transformation, Bacterial
Virulence
Virulence - genetics
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Summary:An avirulent wild-type avian Escherichia coli strain (Av) was electrotransformed with plasmids coding for complement resistance (pKT107) and Colicin V (ColV) production (pHK11) in order to study the effects of complement resistance and ColV production on virulence. Transformants were also compared with the wild type for embryo lethality, uptake by macrophages, motility, growth rate, plasmid content, and hemolysis. Growth rates and complement resistance patterns of strain Av and transformant Av + pHK11 were similar, but Av + pHK11 caused a significantly greater number of deaths in embryos and acquired motility. Transformant Av + pKT107 had a lower rate of phagocytosis, a slower growth rate, and a greater sensitivity to complement, and it changed from being non-hemolytic to expressing alpha-hemolytic action. The 35-kb plasmid present in the wild type was not present in the transformants. Although some of the results demonstrate the difficulties encountered in using wild-type organisms as recipients in virulence studies, the results with Av + pHK11 indicate that ColV production plus the acquisition of motility contributes to the virulence of avian E. coli
ISSN:0005-2086
1938-4351
DOI:10.2307/1591845