Adaptation of the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay for the determination of virus-neutralizing antibodies using the virus-neutralization assay

The tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] has been widely used for bioassays. Herein is described the use of the MTT dye with a virus-neutralization (VN) assay to titer infectious bursal disease virus (IBDV) neutralizing antibodies. A standard VN assay...

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Bibliographic Details
Published in:Avian diseases 1994-10, Vol.38 (4), p.755-761
Main Authors: Haddad, E.E. (EMBREX, Inc., Research Triangle Park, NC.), Whitfill, C.E, Ricks, C.A, Fredericksen, T, Rowe, D, Owen, L, Baldridge, A, Murray, L, Thoma, J.A
Format: Article
Language:English
Subjects:
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
Animals
Antibodies
Antibodies, Viral - blood
ANTICORPS
ANTICUERPOS
Bioassay
Birnaviridae Infections - immunology
Birnaviridae Infections - veterinary
Birnaviridae Infections - virology
Bromides
Cell lines
Cells, Cultured
Chick Embryo
Chickens - immunology
Chickens - virology
COLORANT
COLORANTES
CULTIVO DE CELULAS
CULTURE DE CELLULE
DOSAGE BIOLOGIQUE
Embryos
ENFERMEDAD DE GUMBORO
ENSAYO BIOLOGICO
FIBROBLASTE
FIBROBLASTOS
Fibroblasts
Geometric lines
Infectious bursal disease virus
Infectious bursal disease virus - immunology
MALADIE DE GUMBORO
Neutralization Tests - veterinary
POLLITO
Poultry Diseases - immunology
Poultry Diseases - virology
POUSSIN
REACCION ANTIGENO-ANTICUERPO
REACCION DE NEUTRALIZACION
REACTION ANTIGENE ANTICORPS
REACTION DE NEUTRALISATION
Sensitivity and Specificity
Tetrazolium Salts
Thiazoles
Titration
Viability
Viruses
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Description
Summary:The tetrazolium salt MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] has been widely used for bioassays. Herein is described the use of the MTT dye with a virus-neutralization (VN) assay to titer infectious bursal disease virus (IBDV) neutralizing antibodies. A standard VN assay using chicken embryo fibroblasts (CEFs) and IBDV was used for the assessment of IBDV-neutralizing antibodies. The percent of CEF killing due to IBDV was quantitated using MTT, and the absorbance (A) data were used to calculate the VN antibody titer. This method of calculation offers the expression of VN titer in terms of units of activity per unit of volume
ISSN:0005-2086
1938-4351
DOI:10.2307/1592111