Phylogenetic relationships among the sections of Clarkia (Onagraceae) inferred from the nucleotide sequences of PgiC

Paralogous genes PgiC1 and PgiC2 resulting from a single duplication encode the cytosolic isozymes of phosphoglucose isomerase (EC 5.3.1.9) in Clarkia. The duplication is ancestral to all extant sections of Clarkia. Both genes are expressed in species with two active PGIC isozymes, but species that...

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Bibliographic Details
Published in:Systematic botany 1996-01, Vol.21 (1), p.45-62
Main Authors: Gottlieb, L.D. (University of California, Davis, CA.), Ford, V.S
Format: Article
Language:English
Subjects:
Amino acids
Biological taxonomies
CITOPLASMA
CODE GENETIQUE
CODIGO GENETICO
CYTOPLASME
DIPLOIDIA
DIPLOIDIE
Diploidy
Exons
EXPRESION GENICA
EXPRESSION DES GENES
FILOGENIA
GENE
GENES
GENETICA
GENETIQUE
Introns
ISOENZIMAS
ISOENZYME
ISOMERASAS
ISOMERASE
Nucleotides
OENOTHERA
ONAGRACEAE
Parsimony
PHYLOGENIE
Phylogeny
Plants
Ravens
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
TAXONOMIA
TAXONOMIE
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Summary:Paralogous genes PgiC1 and PgiC2 resulting from a single duplication encode the cytosolic isozymes of phosphoglucose isomerase (EC 5.3.1.9) in Clarkia. The duplication is ancestral to all extant sections of Clarkia. Both genes are expressed in species with two active PGIC isozymes, but species that have a single PGIC isozyme have an expressed PgiC1 and a silenced psi PgiC2. Genomic nucleotide sequences have been obtained for eight PgiC1 and five PgiC2 genes from species representing six of the eight presently recognized diploid sections of Clarkia. The sequences include a nearly complete psi PgiC2 from C. mildrediae and a partial psi PgiC2 from C. rostrata. Parsimony analysis using the single PgiC of Oenothera mexicana as outgroup places the sequences of Clarkia into two groups corresponding to PgiC1 and PgiC2. The tree is strongly supported by bootstrapping and the modified Templeton's test. Fitch-Margoliash and neighbor-joining methods give the same tree. The results for the two genes are in perfect accord and establish the relationships among the sections. From the base of the genus, the sections branch in the following order: sect. Eucharidium, sect. Rhodanthos, sect. Myxocarpa, sect. Phaeostoma, sect. Godetia, and sect. Sympherica. The only ambiguity concerns whether the basal lineage is sect. Eucharidium alone or paired with sect. Rhodanthos. Data from PgiC1 alone provide 92.5% bootstrap support for the basal placement of sect. Eucharidium. The phylogenetic tree reveals that PgiC2 has been silenced at least four times independently. The results demonstrate the value of nuclear PgiC sequences to resolve difficult phylogenetic problems
ISSN:0363-6445
1548-2324
DOI:10.2307/2419562