Effects of abscisic acid and ammonium ion on morphogenesis of cultured barley embryos

Excised, immature embryos of barley, Hordeum vulgare L., cv. Moore, were cultured upon nutrient agar media containing abscisic acid (ABA) and ammonium ion (NH4 +) in several combinations and concentrations. NH4 +was growth promoting in the range 2.1 to 8.6 mM. ABA, in combination with NH4 +, inhibit...

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Bibliographic Details
Published in:Bulletin of the Torrey Botanical Club 1979-04, Vol.106 (2), p.110-116
Main Authors: Umbeck, Paul F., Norstog, Knut
Format: Article
Language:English
Subjects:
Barley
Coleoptiles
Culture media
Embryos
Germination
Immatures
Ligules
Plant growth
Plants
Quaternary ammonium compounds
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Summary:Excised, immature embryos of barley, Hordeum vulgare L., cv. Moore, were cultured upon nutrient agar media containing abscisic acid (ABA) and ammonium ion (NH4 +) in several combinations and concentrations. NH4 +was growth promoting in the range 2.1 to 8.6 mM. ABA, in combination with NH4 +, inhibited coleoptile and scutellum elongation but promoted differentiation of the scutellum and had a normalizing effect on overall morphology. Embryos, wounded by a longitudinal cut through the shoot apex, underwent a partial regeneration in vitro. This was more pronounced when embryos were cultured upon media containing ABA.
ISSN:0040-9618
2325-8055
DOI:10.2307/2484285