The Intradermal Test in the Diagnosis of Schistosomiasis mansoni. IX. Skin Response to a Purified Fraction Isolated from Cercarial Extracts

Butanol powder from lyophilized cercariae (Schistosoma mansoni) was extracted either with 0.9 NaCl or 0.1 m borate buffer pH 8.0, precipitated at pH 4.7 and the supernatant (total extract) fractionated by column chromatography (DEAE-Sephadex). The fractions obtained were assayed for protein and poly...

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Bibliographic Details
Published in:The Journal of parasitology 1965-10, Vol.51 (5), p.753-756
Main Authors: Gazzinelli, G., Ramalho-Pinto, F. J., Pellegrino, J., J. M. Pompeu Memória
Format: Article
Language:English
Subjects:
Antigens
Cercariae
Chromatography
Fractions
Intradermal tests
Polysaccharides
Probabilities
Reagents
Schistosomiasis
Skin tests
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Summary:Butanol powder from lyophilized cercariae (Schistosoma mansoni) was extracted either with 0.9 NaCl or 0.1 m borate buffer pH 8.0, precipitated at pH 4.7 and the supernatant (total extract) fractionated by column chromatography (DEAE-Sephadex). The fractions obtained were assayed for protein and polysaccharide and skin tested in patients with active schistosomiasis mansoni. Several fractions were found to elicit a skin response. One of them (fraction D, in which no polysaccharide was detected) was at least five times more active than the total extract and contained only 20% of the absorbing material at 280 mμ.
ISSN:0022-3395
1937-2345
DOI:10.2307/3276151