The Effects of Ionizing Radiations on Endocrine Cells: VI. Alterations in Androgen Biosynthesis by Canine Testicular Tissue after the Internal Deposition of Some Radionuclides

Male beagle dogs were injected intravenously with various radionuclides (^{226}{\rm Ra},{}^{239}{\rm Pu},{}^{228}{\rm Th},{}^{228}{\rm Ra}$, and 90 Sr) when approximately 18 months of age. At various time intervals the testes were removed from the animals and incubated with $\text{pregnenolone-}7\al...

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Bibliographic Details
Published in:Radiation research 1967-11, Vol.32 (3), p.520-537
Main Authors: Ellis, Legrande C., Berliner, David L.
Format: Article
Language:English
Subjects:
Androgens
Androgens - biosynthesis
Animals
Autopsies
Biosynthesis
Canines
Carbon Isotopes
Dogs
Injections, Intravenous
Lesions
Male
Plutonium
Pregnenolone - metabolism
Progesterone - metabolism
Radiation Effects
Radionuclides
Radium
Steroids
Strontium Isotopes
Testes
Testis - radiation effects
Testosterone
Thorium
Tritium
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Summary:Male beagle dogs were injected intravenously with various radionuclides (^{226}{\rm Ra},{}^{239}{\rm Pu},{}^{228}{\rm Th},{}^{228}{\rm Ra}$, and 90 Sr) when approximately 18 months of age. At various time intervals the testes were removed from the animals and incubated with $\text{pregnenolone-}7\alpha \text{-}{}^{3}{\rm H}$ and $\text{progesterone-}4\text{-}{}^{14}{\rm C}$. The resulting steroid intermediates were isolated by paper chromatography and derivative formation. The pathways observed for the synthesis of androgens were essentially the same as those observed from perfusion studies by other workers. The 17α-hydroxylation of progesterone was diminished in all but one animal, which had received 90 Sr. Similarly, less androstenedione, testosterone, and total androgens were produced by the tissues from the treated animals as compared to the control group. Pregnanedione was isolated from the incubations of at least eight dogs. The occurrence of this compound with respect to fevers in treated animals was discussed. When a NADPH-generating system was added to incubation of tissues from both groups of animals, there was an increase in steroid biotransformations for both groups. Although the increase in millimicromoles of testosterone and androstenedione production was greater for the control group after the addition of the cofactor-generating system, the percentage of increase in androstenedione, testosterone, and total androgen production was greater for the treated group.
ISSN:0033-7587
1938-5404
DOI:10.2307/3572264